pFUGW mCherry-KASH

LOCUS       pFUGW mCherry-KASH               10190 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  mCherry_KASH epression under hUBC promotor; serves as backbone for 
            ORANGE lenti knock-in.
ACCESSION   .
VERSION     .
KEYWORDS    pFUGW mCherry-KASH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, 
            Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
  TITLE     ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope 
            tagging of endogenous proteins in neurons.
  JOURNAL   PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 
            10.1371/journal.pbio.3000665.
  PUBMED    32275651
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    247..364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        429..1640
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1477..1496
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1687..2397
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MAVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGAL
                     KGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYER
                     AEGRHSTGGMDELYK"
     primer_bind     complement(1835..1853)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2103..2122
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2689..3277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2742..2762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3160..3171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3280..3296)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3281..3297)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3802..3982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4008..4025)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4014..4238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4284..4712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4371..4390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4581..4602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4721..4741)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4726..5055
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4906..5041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4968..4987
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5103..5150
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5169..5543
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5673..5794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5764..5783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5843..5859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5843..5859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5856..5878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5867..5883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5891..5921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5936..5957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6074..6091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6245..6833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6325..6344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7004..7864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7627..7646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7865..7969)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8044..8063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8235..8614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8616..8814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8769..8789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8832..9012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9059..9184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9677..9910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10095..10139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
       61 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
      121 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
      181 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
      241 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      361 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaacc cgtgtcggct
      421 ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
      481 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
      541 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      601 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      661 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      721 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      781 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
      841 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
      901 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
      961 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     1021 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     1081 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     1141 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     1201 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     1261 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     1321 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     1381 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     1441 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     1501 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     1561 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     1621 ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg atccccgggt
     1681 accggtatgg cggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1741 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1801 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1861 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1921 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1981 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2041 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2101 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2161 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2221 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2281 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2341 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     2401 ggactcagat ctcgagagga ggaggaggag acagacagca ggatgcccca cctcgacagc
     2461 cccggcagct cccagccgag acgctccttc ctctcaaggg tgatcagggc agcgctaccg
     2521 ttgcagctgc ttctgctgct gctgctgctc ctggcctgcc tgctacctgc ctctgaagat
     2581 gactacagct gcacccaggc caacaacttt gcccgatcct tctaccccat gctgcggtac
     2641 accaacgggc cacctcccac ctaggaattc gatatcaagc ttatcgataa tcaacctctg
     2701 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2761 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2821 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2881 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2941 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3061 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3121 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3181 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3241 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     3301 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     3361 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     3421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3481 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     3541 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     3601 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     3661 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     3721 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     3781 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3961 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4021 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4081 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4141 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4201 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4261 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4621 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4681 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4801 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4861 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4921 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4981 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5041 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5101 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5161 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5221 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     5281 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     5341 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     5401 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     5461 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     5521 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     5581 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5641 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7981 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8041 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8101 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8161 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8221 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     8281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     8341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     8401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     8461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     8521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     8581 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     8641 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     8701 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     8761 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     8821 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8881 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8941 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9001 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9061 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9121 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9181 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9241 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9301 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9361 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9421 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9481 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     9541 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9601 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9661 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9721 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9781 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9841 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9901 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     9961 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10021 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10081 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10141 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
//